evans blue dye saline solution  (Beijing Solarbio Science)

Journal:

Article Title: Acute Kidney Injury Leads to Inflammation and Functional Changes in the Brain

doi: 10.1681/ASN.2007080901

Figure Lengend Snippet: Brain water content (A) and Evans blue extravasations (B). All mice underwent either 60 min of bilateral renal ischemia followed by reperfusion or underwent a sham operation. The brains were harvested at 24 h after surgery and accessed for water content or for Evans blue dye extravasations. As a positive control, water content and vascular permeability were increased in the ischemic kidney as expected. There was a significant increase in Evans blue extravasations and a comparable level in water content in the brain from the mice with AKI when compared with sham-operated mice. (A) Brain water content index, sham versus AKI, 0.216 ± 0.007 versus 0.231 ± 0.005, P = 0.10. (B) Brain Evans blue dye extravasations in μg/g tissue, sham versus AKI, 40.7 ± 6.29 versus 58.6 ± 7.13, *P = 0.034, n = 3. In the kidney, **P < 0.001 versus sham, n = 4.

Article Snippet: 43 Thirty minutes before the mice were killed, 1% of Evans blue dye saline solution (Sigma Chemical Co., St. Louis, MO) was injected via right jugular vein at 2.5 ml/kg body wt while the mice received light ether anesthesia.

Techniques: Positive Control, Permeability